{"id":24,"date":"2015-02-23T16:44:26","date_gmt":"2015-02-23T07:44:26","guid":{"rendered":"http:\/\/www.sci.hokudai.ac.jp\/CSF2-web\/?page_id=24"},"modified":"2024-09-17T17:52:20","modified_gmt":"2024-09-17T08:52:20","slug":"theme03","status":"publish","type":"page","link":"https:\/\/c-mng.cwh.hokudai.ac.jp\/biol.sci\/Root\/research\/theme03","title":{"rendered":"\u6804\u990a\u30b9\u30c8\u30ec\u30b9\u9069\u5fdc\u306e\u9375\u56e0\u5b50ATL31\u306814-3-3\u306e\u6a5f\u80fd\u89e3\u6790"},"content":{"rendered":"

\u6804\u990a\u7d20\u3068C\/N\u30d0\u30e9\u30f3\u30b9\u3068\u306f\uff1f<\/h1>\n

\u504f\u98df\u3084\u904e\u98df\u306b\u3088\u308b\u4f53\u5185\u6804\u990a\u30d0\u30e9\u30f3\u30b9\u306e\u4e71\u308c\u304c\u539f\u56e0\u3068\u306a\u308a\u5f15\u304d\u8d77\u3053\u3055\u308c\u308b\u300c\u30e1\u30bf\u30dc\u30ea\u30c3\u30af\u30b7\u30f3\u30c9\u30ed\u30fc\u30e0\u300d\u306f\uff0c\u73fe\u4ee3\u4eba\u306e\u5927\u304d\u306a\u95a2\u5fc3\u4e8b\u306e\u4e00\u3064\u3067\u3059\u3002
\n\u540c\u69d8\u306b\uff0c\u690d\u7269\u304c\u751f\u5b58\u3059\u308b\u3046\u3048\u3067\u3082\u6804\u990a\u306e\u30d0\u30e9\u30f3\u30b9\u3092\u4fdd\u3064\u3053\u3068\u306f\u91cd\u8981\u3067\u3059\u3002\u305f\u3060\u3057\uff0c\u4eba\u9593\u304c\u6804\u990a\u6e90\u3068\u306a\u308b\u98df\u3079\u7269\u3092\u81ea\u5206\u3067\u9078\u629e\u3067\u304d\u308b\u306e\u306b\u5bfe\u3057\u3066\uff0c\u5730\u8868\u306b\u56fa\u5b9a\u3055\u308c\u52d5\u3051\u306a\u3044\u690d\u7269\u306f\uff0c\u81ea\u3089\u304c\u751f\u5b58\u3057\u3066\u3044\u308b\u5834\u6240\u3067\u306e\u6804\u990a\u74b0\u5883\u306b\u9069\u5fdc\u3057\u3066\u3044\u304b\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002
\n\u7279\u306b\uff0c\u70ad\u7d20\u6e90\uff08C\uff09\u3067\u3042\u308b\u7cd6\u3068\u4e3b\u8981\u6804\u990a\u7d20\u3067\u3042\u308b\u7a92\u7d20\uff08N\uff09\u306e\u7372\u5f97\u306f\u690d\u7269\u306e\u767a\u751f\u30fb\u6210\u9577\u3092\u5927\u304d\u304f\u5de6\u53f3\u3057\u307e\u3059\u3002\u3053\u308c\u3089\u306f\uff0c\u30bf\u30f3\u30d1\u30af\u8cea\u5408\u6210\u3092\u4ee3\u8868\u3068\u3057\u3066\uff0c\u7269\u8cea\u4ee3 \u8b1d\u306e\u5168\u4f53\u306b\u304a\u3044\u3066\u5bc6\u63a5\u306b\u95a2\u308f\u308a\u3042\u3063\u3066\u3044\u307e\u3059\u3002\u3064\u307e\u308a\u690d\u7269\u306f\uff0c\u5149\u5408\u6210\u306b\u3088\u308a\u8449\u3067\u4f5c\u308a\u51fa\u3055\u308c\u305f\u7cd6\u3068\uff0c\u6839\u304b\u3089\u5438\u53ce\u3057\u305f\u7a92\u7d20\u6e90\u3092\u3088\u308a\u52b9\u7387\u3088\u304f\u5206\u914d\u3057\uff0c\u4ee3\u8b1d\u306b\u5229\u7528\u3059 \u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\uff0c\u690d\u7269\u306f\uff0c\u70ad\u7d20\u3068\u7a92\u7d20\u306e\u4ee3\u8b1d\u8abf\u7bc0\u306b\u95a2\u3059\u308b\u500b\u5225\u306e\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u6a5f\u69cb\u306b\u52a0\u3048\u3066\uff0c\u7d30\u80de\u5185\u306e\u70ad\u7d20\u304a\u3088\u3073\u7a92\u7d20\u4ee3\u8b1d\u7269\u306e\u76f8\u5bfe\u91cf\u6bd4\uff08C\/N\u30d0 \u30e9\u30f3\u30b9\uff09\u3092\u611f\u77e5\u3057\uff0c\u74b0\u5883\u306b\u9069\u5fdc\u3059\u308b\u80fd\u529b\uff0c\u3059\u306a\u308f\u3061C\/N\u5fdc\u7b54\u6a5f\u69cb\u3092\u5099\u3048\u3066\u3044\u307e\u3059\u3002\u300cC\/N\u300d\u306f\u7d30\u80de\u5185\u306e\u57fa\u5e79\u4ee3\u8b1d\uff0c\u3055\u3089\u306b\u306f\u690d\u7269\u306e\u30e9\u30a4\u30d5\u30b5\u30a4\u30af\u30eb\u8ee2\u63db\u3092\u5236\u5fa1\u3059\u308b\u91cd\u8981\u306a\u30b7\u30b0\u30ca\u30eb\u3068\u306a\u308a\u307e\u3059\u3002\u307e\u305f\u8fd1\u5e74\uff0c\u4e16\u754c\u898f\u6a21\u3067\u306e\u74b0\u5883\u554f\u984c\u3067\u3082\u3042\u308b\u5927\u6c17\u4e2dCO2<\/sub>\u6fc3\u5ea6\u5897\u52a0\u3068\u3044\u3046\u89b3\u70b9\u304b\u3089\u3082\uff0cC\/N\u306b\u3088\u308b\u690d\u7269\u306e\u6210\u9577\u5236\u5fa1\u304c\u6ce8\u76ee\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u56f31\uff09\u3002<\/p>\n

\"\u56f31\"<\/a>

\u56f31\u3000\u690d\u7269\u306eC\/N\u5fdc\u7b54<\/p><\/div>\n

C\/N\u30d0\u30e9\u30f3\u30b9\u304c\u690d\u7269\u306e\u6210\u9577\u3092\u5236\u5fa1\u3057\u3066\u3044\u308b\u4f8b\u3092\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u3067\u793a\u3057\u307e\u3057\u3087\u3046\uff08\u56f3\uff12\uff09\u3002
\n\u9ad8\u3044\u7cd6\u6fc3\u5ea6\u304b\u3064\u4f4e\u3044\u7a92\u7d20\u6fc3\u5ea6 \uff08\u9ad8C\/\u4f4eN\uff09 \u6761\u4ef6\u4e0b\u3067\u306f\u690d\u7269\u306e\u751f\u80b2\u306f\u8457\u3057\u304f\u963b\u5bb3\u3055\u308c\u307e\u3059\u304c\uff0c\u540c\u3058\u7cd6\u6fc3\u5ea6\u3067\u3082\uff0c\u305d\u3053\u306b\u7a92\u7d20\u3092\u6dfb\u52a0\u3059\u308b\u3053\u3068\u3067\u751f\u80b2\u963b\u5bb3\u306f\u7de9\u548c\u3055\u308c\u307e\u3059\uff08\u9ad8C\/\u9ad8N\uff09\u3002\u9006 \u306b\uff0c\u540c\u3058\u4f4e\u7a92\u7d20\u6fc3\u5ea6\u3067\u3082\u7cd6\u306e\u91cf\u3092\u6e1b\u3089\u3059\u3053\u3068\u3067\u540c\u69d8\u306e\u30b9\u30c8\u30ec\u30b9\u7de9\u548c\u52b9\u679c\u304c\u307f\u3089\u308c\u307e\u3059\uff08\u4f4eC\/\u4f4eN\uff09\u3002\u3064\u307e\u308a\uff0cC\u3068N\u305d\u308c\u305e\u308c\u5358\u72ec\u306e\u5b58\u5728\u91cf\u3067\u306f\u306a\u304f\uff0c\u76f8\u5bfe\u7684\u306aC\/N\u30d0\u30e9\u30f3\u30b9\u304c\u690d\u7269\u306e\u767a\u82bd\u5f8c\u6210\u9577\u3092\u5236\u5fa1\u3059\u308b\u91cd\u8981\u306a\u56e0\u5b50\u306b\u306a\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002<\/p>\n

\"\u56f32\"<\/a>

\u56f32\u3000C\/N\u306b\u5fdc\u7b54\u3057\u305f\u767a\u82bd\u5f8c\u6210\u9577\u306e\u5236\u5fa1<\/p><\/div>\n

C\/N\u306f\u767a\u82bd\u5f8c\u6210\u9577\u3060\u3051\u3067\u306f\u306a\u304f\uff0c\u6804\u990a\u6210\u9577\u76f8\u304b\u3089\u751f\u6b96\u6210\u9577\u76f8\u3078\u306e\u8ee2\u63db\u70b9\u3067\u3042\u308b\u300c\u82b1\u6210\u300d\u3084\u8001\u5316\u3068\u3044\u3063\u305f\u690d\u7269\u306e\u30e9\u30a4\u30d5\u30b5\u30a4\u30af\u30eb\u5168\u822c\u306e\u30c1\u30a7\u30c3\u30af\u30dd\u30a4\u30f3\u30c8\u306b\u304a\u3044\u3066\u3082\u91cd\u8981\u306a\u56e0\u5b50\u3067\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u3063\u3066\u304d\u3066\u3044\u307e\u3059\uff08\u56f33\uff09\u3002
\n\u307e\u305f\uff0c\u82b1\u6210\u3084\u8001\u5316\u306e\u5236\u5fa1\u306b\u304a\u3044\u3066\u306f\u300c\u9ad8C\/\u4f4eN\u300d\u3067\u3088\u308a\u4fc3\u9032\u7684\u306a\u52b9\u679c\u3092\u6709\u3059\u308b\u3053\u3068\u304c\u5206\u304b\u3063\u3066\u304d\u3066\u304a\u308a\uff0c\u767a\u82bd\u5f8c\u6210\u9577\u3068\u306f\u9006\u306b\u306a\u308b\u3053\u3068\u3082\u8208\u5473\u6df1\u3044\u70b9\u3067\u3059\u3002<\/p>\n

\"\u56f33\"<\/a>

\u56f33\u3000C\/N\u306b\u3088\u308b\u690d\u7269\u306e\u30e9\u30a4\u30d5\u30b5\u30a4\u30af\u30eb\u5236\u5fa1<\/p><\/div>\n

C\/N\u304c\u690d\u7269\u306e\u767a\u82bd\u5f8c\u6210\u9577\u3084\u82b1\u6210\u3068\u3044\u3063\u305f\u6210\u9577\u76f8\u3092\u5236\u5fa1\u3059\u308b\u56e0\u5b50\u3068\u3057\u3066\u91cd\u8981\u306a\u3053\u3068\u306f\u53e4\u304f\u304b\u3089\u77e5\u3089\u308c\u3066\u304a\u308a\uff0c\u8fb2\u696d\u7684\u306a\u89b3\u70b9\u3067\u3082\u5229\u7528\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002
\n\u305d\u306e\u4e00\u65b9 \u3067\uff0c\u305d\u306e\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u6a5f\u69cb\u306e\u5206\u5b50\u5b9f\u4f53\u306b\u3064\u3044\u3066\u306f\u672a\u3060\u306b\u8b0e\u306e\u307e\u307e\u3067\u3057\u305f\u3002<\/p>\n

C\/N\u5fdc\u7b54\u5236\u5fa1\u306e\u5206\u5b50\u30e1\u30ab\u30cb\u30ba\u30e0\u3068\u306f\uff1f<\/h1>\n

\u3053\u308c\u307e\u3067\u306e\u7814\u7a76\u304b\u3089\uff0c\u690d\u7269\u306b\u304a\u3051\u308bC\/N\u30d0\u30e9\u30f3\u30b9\u30bb\u30f3\u30b5\u30fc\u5019\u88dc\u3068\u3057\u3066\uff0c2-\u30aa\u30ad\u30bd\u30b0\u30eb\u30bf\u30eb\u9178\uff082-OG\uff09\u7d50\u5408\u6d3b\u6027\u3092\u6709\u3059\u308b\u5927\u8178\u83ccPII\u5206\u5b50\u306e\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u30db\u30e2\u30ed\u30b0\u3067\u3042\u308bAtGLB1\u3084\u30b0\u30eb\u30bf\u30df\u30f3\u9178\u53d7\u5bb9\u4f53\u3067\u3042\u308bAtGLR1.1\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3057\u305f\u3002
\n2-OG\u306f\u30af\u30a8\u30f3\u9178\u56de\u8def\u306e\u4ee3\u8b1d\u4e2d\u9593\u4f53\u3067\u3042\u308a\uff0c\u307e\u305f\u76f4\u63a5\u30a2\u30df\u30ce\u9178\u5408\u6210\u7d4c\u8def\u306b\u5229\u7528\u3055\u308c\u308b\u5316\u5408\u7269\u3067\u3059\u3002\u6839\u3067\u5438\u53ce\u3055\u308c\u305f\u7a92\u7d20\u6e90\u306f\uff0c\u30b0\u30eb\u30bf\u30df\u30f3\uff0d\u30b0\u30eb\u30bf\u30df\u30f3\u9178\u5408\u6210 \u56de\u8def\u3092\u7d4c\u3066\uff0c\u30a2\u30df\u30ce\u9178\u306b\u5408\u6210\u3055\u308c\u307e\u3059\u30022-OG\u306f\uff0c\u305d\u306e\u969b\uff0c\u30a2\u30df\u30ce\u57fa\u3092\u53d7\u5bb9\u3059\u308b\u70ad\u7d20\u9aa8\u683c\u3068\u3057\u3066\u5229\u7528\u3055\u308c\u307e\u3059\u3002\u5f93\u3063\u3066\uff0c\u3053\u308c\u30892\u3064\u306e\u5019\u88dc\u5206\u5b50\u306f\u3044\u305a\u308c\u3082\u70ad\u7d20 \u4ee3\u8b1d\u3068\u7a92\u7d20\u4ee3\u8b1d\u7d4c\u8def\u306e\u76f8\u4e92\u4f5c\u7528\u70b9\u306b\u95a2\u308f\u308b\u5206\u5b50\u3068\u3057\u3066\u6ce8\u76ee\u3055\u308c\u3066\u3044\u307e\u3057\u305f\u3002\u5927\u8178\u83cc\u306b\u304a\u3044\u3066\u306f\uff0cPII\u30bf\u30f3\u30d1\u30af\u8cea\u304cC\/N\u30bb\u30f3\u30b5\u30fc\u3068\u3057\u3066\u6a5f\u80fd\u3059\u308b\u3068\u8003\u3048\u3089\u308c\u3066 \u3044\u307e\u3059\u3002\u3064\u307e\u308a\uff0cAtGLB1\u304a\u3088\u3073AtGLR1.1\u306f\u3044\u305a\u308c\u3082\u4ee3\u8b1d\u7684\u5074\u9762\u3042\u308b\u3044\u306f\u4ed6\u306e\u751f\u7269\u7a2e\u3067\u306e\u7814\u7a76\u304b\u3089\u305d\u306e\u91cd\u8981\u6027\u304c\u4e88\u60f3\u3055\u308c\u305f\u3082\u306e\u3067\u3059\u3002
\n\u690d\u7269\u306b\u304a\u3044\u3066\u3082\u4e21\u907a\u4f1d\u5b50\u3092\u5bfe\u8c61\u3068\u3057\u305f\u907a\u4f1d\u5b66\u7684\u89e3\u6790\u304c\u884c\u308f\u308c\u3066\u304d\u307e\u3057\u305f\u304c\uff0cC\/N\u30bb\u30f3\u30b5\u30fc\u3068\u3057\u3066\u306e\u691c\u8a3c\u306f\u672a\u3060\u4e0d\u5b8c\u5168\u306a\u307e\u307e\u3067\u3059\u3002\u5f93\u3063\u3066\uff0c\u690d\u7269\u306b\u304a\u3051\u308bC \/N\u5fdc\u7b54\u6a5f\u69cb\u306e\u5b9f\u614b\u306b\u3064\u3044\u3066\u306f\u591a\u304f\u306e\u8b0e\u304c\u6b8b\u3055\u308c\u305f\u307e\u307e\u3067\u3057\u305f\u3002\u305d\u306e\u305f\u3081\uff0c\u65b0\u305f\u306aC\/N\u30bb\u30f3\u30b5\u30fc\u304a\u3088\u3073\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u5206\u5b50\u5019\u88dc\u306e\u767a\u898b\u304c\u5f85\u305f\u308c\u3066\u3044\u307e\u3057\u305f\u3002
\n\u79c1\u305f\u3061\u306e\u7814\u7a76\u304b\u3089\uff0c\u690d\u7269C\/N\u5fdc\u7b54\u5236\u5fa1\u6a5f\u69cb\u306b\u304a\u3044\u3066\u300c\u30e6\u30d3\u30ad\u30c1\u30f3\u30ab\u30b9 \u30b1\u30fc\u30c9\u2010\u30d7\u30ed\u30c6\u30a2\u30bd\u30fc\u30e0\u30b7\u30b9\u30c6\u30e0\uff08\u4ee5\u4e0bUPS\uff09\u300d\u3092\u4ecb\u3057\u305f\u80fd\u52d5\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u304c\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3059\u3053\u3068\u304c\u767a\u898b\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n

C\/N\u5fdc\u7b54\u7570\u5e38\u5909\u7570\u4f53\u306e\u767a\u898b
\n\uff5e\u30e6\u30d3\u30ad\u30c1\u30f3\u2010\u30d7\u30ed\u30c6\u30a2\u30bd\u30fc\u30e0\u30b7\u30b9\u30c6\u30e0\u306b\u3088\u308b\u6804\u990a\u5fdc\u7b54\u5236\u5fa1\uff5e<\/h1>\n

\u305d\u3053\u3067\u79c1\u305f\u3061\u306e\u7814\u7a76\u5ba4\u3067\u306f\uff0c\u65b0\u898fC\/N\u5fdc\u7b54\u7570\u5e38\u5909\u7570\u4f53\u306e\u5358\u96e2\u3092\u76ee\u6307\u3057\uff0c\u72ec\u81ea\u306eC\/N\u30b9\u30c8\u30ec\u30b9\u57f9\u5730\u3092\u7528\u3044\u305f\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3092\u884c\u3063\u3066\u304d\u307e\u3057\u305f\u3002
\n\u7406\u5316\u5b66\u7814\u7a76\u6240\u690d\u7269\u79d1\u5b66\u30bb\u30f3\u30bf\u30fc\u304c\u958b\u767a\u3057\u305f\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u6a5f\u80fd\u7372\u5f97\u578b\u5909\u7570\u4f53FOX\uff08Full-length cDNA OvereXpressor\uff09\u30e9\u30a4\u30f3\u3092\u6750\u6599\u306b\uff0c\u3053\u308c\u307e\u3067\uff0c\u7d046,000\u30e9\u30a4\u30f3\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3092\u884c\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\uff0c\u3053\u306e\u57f9\u5730\u3067\u8010\u6027\u3092\u793a\u3059C\/N\u5fdc \u7b54\u7570\u5e38\u5909\u7570\u4f53”carbon\/nitrogen insensitive 1-D <\/em>(cni1-D<\/em>)\u201d<\/em>\u304c\u5f97\u3089\u308c\u307e\u3057\u305f\u3002cni1-D<\/em>\u5909\u7570\u4f53\u306f\uff0c\u91ce\u751f\u578b\u690d\u7269\u304c\u67af\u6b7b\u3059\u308b\u3088\u3046\u306a\u9ad8C\/\u4f4eN\u30b9\u30c8\u30ec\u30b9\u6761\u4ef6\u3067\u3082\u5b50\u8449\u306e\u7dd1\u5316\u304c\u89b3\u5bdf\u3055\u308c\uff0cC\/N\u30b9\u30c8\u30ec\u30b9\u8010\u6027\u3092\u793a\u3057\u307e\u3057\u305f\uff08\u56f34\uff09\u3002\u4e0a\u8a18\u5909\u7570\u4f53\u306f\u539f\u56e0\u907a\u4f1d\u5b50CNI1<\/em>\u306e\u904e\u5270\u767a\u73fe\u304c\u539f\u56e0\u3067\u3057\u305f\u304c\uff0c\u3053\u306e\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u6b20\u640d\u5909\u7570\u4f53\u3067\u306f\uff0c\u9006\u306bC\/N\u30b9\u30c8\u30ec\u30b9\u306b\u904e\u5270\u5fdc\u7b54\u3092\u793a\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u304b\u3089\uff0cCNI1<\/em>\u306fC\/N\u5fdc\u7b54\u5236\u5fa1\u5206\u5b50\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u307e\u3057\u305f\u3002\u539f\u56e0\u907a\u4f1d\u5b50CNI1<\/em>\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u540c\u5b9a\u3057\u305f\u7d50\u679c\uff0cRING-H2\u578b\u306e\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc\uff08E3\uff09\u3067\u3042\u308bATL31\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30b3\u30fc\u30c9\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u5224\u308a\u307e\u3057\u305f\u3002<\/p>\n

\"cni1-Djpg\"<\/a>

\u56f34\u3000\u65b0\u5947C\/N\u5fdc\u7b54\u7570\u5e38\u5909\u7570\u4f53cni1-D\u306e\u5358\u96e2<\/p><\/div>\n

\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc\u306fUPS\u306e\u9375\u3068\u306a\u308b\u5206\u5b50\u3067\u3059\u3002\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc\u306f\uff0c\u5206\u89e3\u3055\u308c\u308b\u3079\u304d\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u76f4\u63a5\u6355\u6349\u3057\uff0cE1\uff0cE2\u3092\u7d4c\u3066\u53d7\u3051\u6e21\u3055\u308c\u305f\u30e6\u30d3\u30ad \u30c1\u30f3\u5206\u5b50\u3092\u6700\u7d42\u7684\u306b\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u4ed8\u52a0\u3057 \u307e\u3059\uff08\u56f32\uff09\u3002\u3064\u307e\u308a\uff0c\u5206\u89e3\u3055\u308c\u308b\u3079\u304d\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7279\u7570\u6027\u3092\u6c7a\u5b9a\u3059\u308b\u975e\u5e38\u306b\u91cd\u8981\u306a\u6a5f\u80fd\u3092\u3082\u3061\uff0cUPS\u3092\u4ecb\u3057\u305f\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u6a5f\u69cb\u306e\u6839\u5e79\u3092\u62c5\u3063\u3066\u3044\u307e\u3059\u3002 \u690d\u7269\u3067\u306f\u5168\u30b2\u30ce\u30e0\u306e5\uff05\u306b\u3042\u305f\u308b\u7d041,200\u907a\u4f1d\u5b50\u304c\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc\u3092\u30b3\u30fc\u30c9\u3057\u3066\u304a\u308a\uff0c\u305d\u306e\u591a\u69d8\u6027\u304c\u690d\u7269\u306e\u3082\u3064\u512a\u308c\u305f\u74b0\u5883\u9069\u5fdc\u80fd\u529b\u306e\u4e00\u7ffc\u3092\u62c5\u3046\u3068\u8003\u3048 \u3089\u308c\u3066\u3044\u307e\u3059\u3002ATL31\u306f\u5b9f\u969b\u306b\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc\u6d3b\u6027\u3092\u6709\u3059\u308b\u3053\u3068\u3082\u78ba\u8a8d\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u307e\u305f\uff0c\u3053\u306eATL31\u306eRING\u30c9\u30e1\u30a4\u30f3\u306b\u5909\u7570\u3092\u5165\u308c\uff0c\u30e6\u30d3\u30ad \u30c1\u30f3 \u6d3b\u6027\u3092\u6b20\u640d\u3055\u305b\u305f\u5834\u5408\uff0c\u305d\u306e\u690d\u7269\u306eC\/N\u8010\u6027\u306f\u5931\u308f\u308c\u307e\u3059\u3002\u3053\u3046\u3057\u305f\u77e5\u898b\u304b\u3089\uff0cATL31\u306f\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc\u3068\u3057\u3066\u690d\u7269C\/N\u5fdc\u7b54\u3092\u5236\u5fa1\u3059\u308b\u5206\u5b50 \u3067\u3042\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u307e\u3057\u305f\uff08\u56f35\uff09\u3002\u307e\u305f\uff0cATL31\u306e\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306fC\/N\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u6a5f\u69cb\u306e\u4e2d\u6838\u3092\u62c5\u3046\u3053\u3068\u304c\u4e88\u60f3\u3055\u308c\u307e\u3057\u305f\u3002\u3064\u307e \u308a\uff0c \u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u540c\u5b9a\u3082\u542b\u3081\u3066\uff0c\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bcATL31\u3068\u305d\u306e\u6d3b\u6027\u5236\u5fa1\u306b\u95a2\u308f\u308b\u5206\u5b50\u306e\u6a5f\u80fd\u89e3\u660e\u306f\uff0c\u9577\u5e74\u4e0d\u660e\u3067\u3042\u3063\u305f\u690d\u7269C\/N\u5fdc\u7b54\u6a5f\u69cb\u306e\u5b9f\u4f53\u89e3\u660e\u306b \u76f4\u7d50\u3059\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059(Sato et al., Plant J, 2009, 60: 852-864)\u3002
\n\u3055\u3089\u306b\uff0c\u3053\u306e\u767a\u898b\u3068\u6642\u671f\u3092\u540c\u3058\u304f\u3057\u3066\uff0c\u7cd6\u3068\u7a92\u7d20\uff0c\u500b\u5225\u306e\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u306b\u95a2\u3057\u3066\u3082UPS\u3092\u4ecb\u3057\u305f\u5236\u5fa1\u6a5f\u69cb\u306e\u5b58\u5728\u304c\u76f8\u6b21\u3044\u3067\u5831\u544a\u3055\u308c\u307e\u3057\u305f\u3002\uff11\u3064\u306f\uff0c\u690d\u7269\u30db\u30eb\u30e2 \u30f3\u3067\u3042\u308b\u30a2\u30d6\u30b7\u30b8\u30f3\u9178\uff08ABA\uff09\u306e\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306b\u95a2\u308f\u308b\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bcKEG\u3067\u3042\u308a\uff0c\u3053\u306e\u5206\u5b50\u306e\u6a5f\u80fd\u6b20\u640d\u5909\u7570\u4f53\u3067\u306f\u7cd6\u306b\u5bfe\u3059\u308b\u5fdc\u7b54\u304a\u3088\u3073\u767a\u82bd\u5f8c\u6210\u9577\u304c \u7570\u5e38\u306b\u306a\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u52a0\u3048\u3066\uff0c\u4f4e\u7a92\u7d20\u6761\u4ef6\u4e0b\u3067\u306e\u751f\u80b2\u304c\u4e0d\u826f\u306b\u306a\u308b\u5909\u7570\u4f53\u306e\u539f\u56e0\u907a\u4f1d\u5b50NLA\u3082\u307e\u305f\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc\u3092\u30b3\u30fc\u30c9\u3057\u3066\u3044\u307e\u3057\u305f\u3002\u4e21 \u5206\u5b50\u306f\u5b9f\u969b\u306b\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc\u6d3b\u6027\u3092\u6709\u3059\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u3066\u304a\u308a\uff0cKEG\u306b\u3064\u3044\u3066\u306f\u65e2\u306bABA\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u306b\u95a2\u308f\u308bABI5\u304c\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u306e\u6a19\u7684\u30bf\u30f3\u30d1\u30af \u8cea\u3068\u3057\u3066\u540c\u5b9a\u3055\u308c\u3066\u3044\u307e\u3059\u3002C\/N\u5fdc\u7b54\u5236\u5fa1\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bcATL31\u306b\u52a0\u3048\u3066\uff0cKEG\u3084NLA\u306e\u767a\u898b\u306b\u3088\u308a\uff0c\u3053\u308c\u307e\u3067\u660e\u3089\u304b\u3068\u306a\u3063\u3066\u3044\u306a\u304b\u3063\u305f\u6804\u990a\u74b0 \u5883 \u306b\u5bfe\u3059\u308b\u5fdc\u7b54\u306b\u304a\u3044\u3066\u3082\uff0cUPS\u304c\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3059\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n

\"\u56f35\"<\/a>

\u56f35\u3000\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bcATL31\u306b\u3088\u308bC\/N\u5fdc\u7b54\u5236\u5fa1\u30e2\u30c7\u30eb<\/p><\/div>\n

\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bcATL31\u3068\u591a\u6a5f\u80fd\u56e0\u5b5014-3-3\u306b\u3088\u308bC\/N\u5fdc\u7b54\u5236\u5fa1<\/h1>\n

\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30c9\u30e1\u30a4\u30f3\u69cb\u9020\u304b\u3089\uff0cCNI1\u306f ATL\u30d5\u30a1\u30df\u30ea\u30fc\u3068\u540d\u3065\u3051\u3089\u308c\u305f\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc\u30d5\u30a1\u30df\u30ea\u30fc\u306e\u4e00\u54e1\uff0cATL31\u3067\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002
\n\u3057\u304b\u3057\uff0cATL31\u306e\u751f\u5316\u5b66\u7684\u6a5f\u80fd\u306f\u672a\u77e5\u306e\u72b6\u614b\u3067\u3042\u308a\uff0cATL31\u306e\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u30bf\u30fc\u30b2\u30c3\u30c8\u306f\u4f55\u304b\uff1f\u3053\u308c\u3092 \u3064\u304d\u3068\u3081\u308b\u3053\u3068\u304c\u6b21\u306e\u91cd\u8981\u8ab2\u984c\u3067\u3057\u305f\u3002<\/p>\n

\u305d\u3053\u3067\uff0cFLAG\u30bf\u30b0\u3092\u7528\u3044\u305f\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u7cbe\u88fd\u3068MS\u89e3\u6790\u306b\u3088\u308bATL31\u76f8\u4e92\u4f5c\u7528\u56e0\u5b50 \u306e\u63a2\u7d22\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u307e\u305f\uff0c\u3088\u308a\u52b9\u679c\u7684\u306b\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u6a19\u7684\u3092\u6355\u6349\u3059\u308b\u305f\u3081\u306b\uff0c\u4e0a\u8ff0\u306eRING\u5909\u7570\u578bATL31\uff08\u4e0d\u6d3b\u6027\u578b\uff09-FLAG\u3092\u767a\u73fe\u3059\u308b\u5f62\u8cea\u8ee2\u63db\u4f53 \u3092\u7528\u3044\u308b\u3053\u3068\u306b\u3057\u307e\u3057\u305f\u3002\u7cbe\u88fd\u7523\u7269\u306eMS\u89e3\u6790\u306e\u7d50\u679c\uff0c\u8907\u6570\u306e14-3-3\u30bf\u30f3\u30d1\u30af\u8cea\u7fa4\u304c\u76f8\u4e92\u4f5c\u7528\u56e0\u5b50\u3068\u3057\u3066\u540c\u5b9a\u3055\u308c\u307e\u3057\u305f\u300214-3-3\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u30ea\u30f3 \u9178\u5316\u3055\u308c\u305f\u591a\u69d8\u306a\u9175\u7d20\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u7d50\u5408\u3057\uff0c\u305d\u306e\u6d3b\u6027\u3092\u5236\u5fa1\u3059\u308b\u5206\u5b50\u3067\u3059\uff08\u56f36\uff09\u3002\u305d\u306e\u4e2d\u3067\u3082\uff0c\u7279\u306bH+-ATPase\u3084\u785d\u9178\u9084\u5143\u9175\u7d20\uff0c\u30b0\u30eb\u30bf\u30df\u30f3\u5408\u6210\u9175\u7d20\uff0c\u30b0\u30eb\u30bf \u30df\u30f3\u9178\u5408\u6210\u9175\u7d20\uff0c\u30b9\u30af\u30ed\u30fc\u30b9\u30ea\u30f3\u9178\u5408\u6210\u9175\u7d20\u306a\u3069\uff0c\u70ad\u7d20\u30fb\u7a92\u7d20\u4ee3\u8b1d\u306e\u4e3b\u8981\u306a\u9175\u7d20\u7fa4\u304c14-3-3\u306e\u7d50\u5408\u306b\u3088\u308a\u6d3b\u6027\u5236\u5fa1\u3092\u53d7\u3051\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u304a\u308a\uff0cC\/N\u5fdc \u7b54\u3068\u306e\u95a2\u9023\u6027\u304c\u975e\u5e38\u306b\u9ad8\u3044\u5236\u5fa1\u56e0\u5b50\u3067\u3059\u3002<\/p>\n

\"14-3-3\"<\/a>

\u56f36\u300014-3-3\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u751f\u5316\u5b66\u7684\u6a5f\u80fd<\/p><\/div>\n

Co-IP\u3084\u9175\u6bcd2\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u7b49\u306e\u89e3\u6790\u306b\u52a0\u3048\uff0c\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7528\u3044\u305fBiFC\uff08Bimolecular Fluorescence Complementation\uff09\u30a2\u30c3\u30bb\u30a4\u306b\u3088\u308a\uff0cATL31\u306814-3-3\u304c\u78ba\u304b\u306b\u690d\u7269\u306e\u7d30\u80de\u819c\u4e0a\u3067\u76f8\u4e92\u4f5c\u7528\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\uff08\u56f37\uff09\u3002\u52a0\u3048 \u3066\uff0cin vitro \u89e3\u6790\u304b\u3089ATL31\u304c\u76f4\u63a5\u7684\u306b14-3-3\u3092\u30dd\u30ea\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u3059\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002\u307e\u305f\uff0c14-3-3\u306f\u5b9f\u969b\u306b\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u306e\u767a\u82bd\u5f8c\u6210\u9577\u6642\u306b ATL31\u5236\u5fa1\u4e0b\u3067C\/N\u30b9\u30c8\u30ec\u30b9\u306b\u5fdc\u3058\u3066\u5b89\u5b9a\u6027\u304c\u5909\u52d5\u3057\uff0c\u3055\u3089\u306b14-3-3\u904e\u5270\u767a\u73fe\u4f53\u304cC\/N\u30b9\u30c8\u30ec\u30b9\u9ad8\u611f\u53d7\u6027\u3092\u793a\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u304b \u3089\uff0cATL31\u306fC\/N\u306b\u5fdc\u3058\u305f14-3-3\u5b89\u5b9a\u6027\u306e\u8abf\u7bc0\u3092\u4ecb\u3057\u3066\uff0c\u690d\u7269\u306e\u767a\u82bd\u5f8c\u6210\u9577\u3092\u5236\u5fa1\u3059\u308b\u3068\u7d50\u8ad6\u3065\u3051\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u3088\u308a\uff0c\u4eca\u307e\u3067\u4e0d\u660e\u3067\u3042\u3063\u305f\u9ad8\u7b49\u690d \u7269\u306eC\/N\u5fdc\u7b54\u5236\u5fa1\u6a5f\u69cb\u306e\u4e00\u7aef\u304c\u660e\u3089\u304b\u306b\u306a\u308a\uff0c\u305d\u3053\u306b\u306f\u30e6\u30d3\u30ad\u30c1\u30f3\uff0d\u30d7\u30ed\u30c6\u30a2\u30bd\u30fc\u30e0\u7cfb\u306b\u3088\u308b\u7279\u7570\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u304c\u95a2\u4e0e\u3059\u308b\u3068\u3044\u3046\u690d\u7269\u72ec\u81ea\u306e\u6804\u990a\u30b9\u30c8\u30ec\u30b9 \u5fdc\u7b54\u6a5f\u69cb\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f(Sato et al., Plant J, 2011, 68:137-146)\u3002<\/p>\n

\"BiFC\"<\/a>

\u56f37\u3000BiFC\u30a2\u30c3\u30bb\u30a4\u306b\u3088\u308bATL31\u306814-3-3\u30bf\u30f3\u30d1\u30af\u8cea\u76f8\u4e92\u4f5c\u7528\u306e\u691c\u51fa<\/p><\/div>\n

\"\u56f38\"<\/a>

\u56f38\u3000ATL31\u306b\u3088\u308b14-3-3\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u3092\u4ecb\u3057\u305fC\/N\u5fdc\u7b54\u5236\u5fa1\u30e2\u30c7\u30eb<\/p><\/div>\n

\u73fe\u5728\u306f\uff0cATL31\u306814-3-3\u306e\u76f8\u4e92\u4f5c\u7528\u306e\u5236\u5fa1\u30e1\u30ab\u30cb\u30ba\u30e0\u3068\u305d\u306e\u4e0a\u6d41\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def \u306b\u3064\u3044\u3066\u89e3\u6790\u3092\u7d9a\u3051\u3066\u3044\u307e\u3059\u3002\u3055\u3089\u306b\uff0c14-3-3\u306e\u4e0b\u6d41\u3067\u5177\u4f53\u7684\u306b\u3069\u3046\u3044\u3063\u305f\u9175\u7d20\u306e\u6d3b\u6027\u30fb\u4ee3\u8b1d\u7269\u91cf\u304c\u5236\u5fa1\u3055\u308c\uff0c\u690d\u7269\u306eC\/N\u5fdc\u7b54\u304c\u8d77\u3053\u308b\u304b\u306b\u3064\u3044\u3066\uff0c\u5148 \u7aef\u7684\u306a\u30d7\u30ed\u30c6\u30aa\u30fc\u30e0\u89e3\u6790\u3084\u30e1\u30bf\u30dc\u30ed\u30fc\u30e0\u89e3\u6790\u3092\u99c6\u4f7f\u3057\u306a\u304c\u3089\uff0c\u5305\u62ec\u7684\u306b\u89e3\u304d\u660e\u304b\u305d\u3046\u3068\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u6804\u990a\u7d20\u3068C\/N\u30d0\u30e9\u30f3\u30b9\u3068\u306f\uff1f \u504f\u98df\u3084\u904e\u98df\u306b\u3088\u308b\u4f53\u5185\u6804\u990a\u30d0\u30e9\u30f3\u30b9\u306e\u4e71\u308c\u304c\u539f\u56e0\u3068\u306a\u308a\u5f15\u304d\u8d77\u3053\u3055\u308c\u308b\u300c\u30e1\u30bf\u30dc\u30ea\u30c3\u30af\u30b7\u30f3\u30c9\u30ed\u30fc\u30e0\u300d\u306f\uff0c\u73fe\u4ee3\u4eba\u306e\u5927\u304d\u306a\u95a2\u5fc3\u4e8b\u306e\u4e00\u3064\u3067\u3059\u3002 \u540c\u69d8\u306b\uff0c\u690d\u7269\u304c\u751f\u5b58\u3059\u308b\u3046\u3048\u3067\u3082\u6804\u990a\u306e\u30d0\u30e9\u30f3\u30b9\u3092\u4fdd\u3064\u3053\u3068\u306f\u91cd\u8981 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":18,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/biol.sci\/Root\/wp-json\/wp\/v2\/pages\/24"}],"collection":[{"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/biol.sci\/Root\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/biol.sci\/Root\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/biol.sci\/Root\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/biol.sci\/Root\/wp-json\/wp\/v2\/comments?post=24"}],"version-history":[{"count":3,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/biol.sci\/Root\/wp-json\/wp\/v2\/pages\/24\/revisions"}],"predecessor-version":[{"id":3669,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/biol.sci\/Root\/wp-json\/wp\/v2\/pages\/24\/revisions\/3669"}],"up":[{"embeddable":true,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/biol.sci\/Root\/wp-json\/wp\/v2\/pages\/18"}],"wp:attachment":[{"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/biol.sci\/Root\/wp-json\/wp\/v2\/media?parent=24"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}